Position “D1F-F1” holds an empty 2 mL screw top vial (p/n ) as waste vial. The vial is capped without a septum to avoid carryover. Draw Draw 80 µL from location “D1F-E1” with default speed using default offset 80 µL of the diluent are drawn into the loop. Position “D1F-E1” holds the diluent in a 2 mL screw top vial