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High-performance liquid chromatography (HPLC) systems are used to analyze and/or purify liquid mixtures. Analytical HPLC systems are used in the food safety, pharmaceutical, and manufacturing industries, as well as in medical and research laboratories. Two of the most common types of analytical HPLC systems are normal phase HPLC and reversed
HPLC Grade Solvents. As the main component of your mobile phase, the solvent you choose needs to be suitable for your application. MilliporeSigma has over 30 years experience producing the highest quality solvents for HPLC applications. Don't trust your work to anything less!
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2016/06/09 · Add your samples into the autosampler tray. Stop the purge. Close the purge valve. Run the system at a normal flow rate (1 ml/min) with your buffer to equilibrate the column for 10 minutes. Make sure that your pressure is stable (ie, less than 2-3 bar of fluctuation) Set up your sequence and your method.
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